| Pos | No | Player 1 | No | Player 2 | No | Player 3 | No | Player 4 | No | Player 5 |
| Offense |
| LWR | 1 |
Samuel Sr., Deebo 19/2
| 18 |
CONLEY, CHRIS U/Ten
| |
| |
| |
|
| RWR | 14 |
Pearsall, Ricky 24/1
| |
| |
| |
| |
|
| SWR | 15 |
Jennings, Jauan 20/7
| 19 |
Cowing, Jacob 24/4
| |
| |
| |
|
| LT | 71 |
WILLIAMS, TRENT T/Was
| 76 |
Moore, Jaylon 21/5
| |
| |
| |
|
| LG | 65 |
Banks, Aaron 21/2
| |
| |
| |
| |
|
| C | 64 |
BRENDEL, JAKE SF20
| 63 |
Zakelj, Nick 22/6
| |
| |
| |
|
| RG | 77 |
Puni, Dominick 24/3
| 74 |
Burford, Spencer 22/4
| |
| |
| |
|
| RT | 68 |
McKivitz, Colton 20/5
| 62 |
Pleasants, Austen SF24
| |
| |
| |
|
| TE | 85 |
KITTLE, GEORGE 17/5
| 82 |
SAUBERT, ERIC U/Hou
| 88 |
Tonges, Jake SF23
| 9 |
Willis, Brayden 23/7
| |
|
| QB | 13 |
Purdy, Brock 22/7
| 17 |
ALLEN, BRANDON U/Cin
| 5 |
Dobbs, Joshua U/Min
| |
| |
|
| RB | 31 |
Guerendo, Isaac 24/4
| 32 |
Taylor Jr., Patrick U/GB
| 49 |
Vaughn, Ke'Shawn SF24
| 47 |
Abanikanda, Israel W/NYJ
| |
|
| FB | 44 |
JUSZCZYK, KYLE U/Bal
| |
| |
| |
| |
|
| Defense |
| LDE | 97 |
Bosa, Nick 19/1
| 94 |
Gross-Matos, Yetur U/Car
| 51 |
Beal Jr., Robert 23/5
| |
| |
|
| LDT | 99 |
Collins, Maliek T/Hou
| 69 |
Anderson, Evan CF24
| |
| |
| |
|
| RDT | 92 |
Elliott, Jordan U/Cle
| 93 |
Davis, Kalia 22/6
| 50 |
Davis, Khalil T/Hou
| |
| |
|
| RDE | 56 |
FLOYD, LEONARD U/Buf
| 91 |
Okuayinonu, Sam SF23
| 58 |
BARRETT, ALEX SF19
| |
| |
|
| WLB | 57 |
Greenlaw, Dre 19/5
| 45 |
Flannigan-Fowles, Demetrius CF19
| |
| |
| |
|
| MLB | 54 |
Warner, Fred 18/3
| 53 |
Winters, Dee 23/6
| 41 |
Graham, Jalen 23/7
| |
| |
|
| LCB | 7 |
Ward, Charvarius U/KC
| 22 |
Yiadom, Isaac U/NO
| |
| |
| |
|
| SS | 29 |
Hufanga, Talanoa 21/5
| 43 |
GIPSON SR., TASHAUN SF24
| |
| |
| |
|
| FS | 6 |
Mustapha, Malik 24/4
| 27 |
Brown, Ji'Ayir 23/3
| |
| |
| |
|
| RCB | 0 |
Green, Renardo 24/2
| 33 |
Ya-Sin, Rock U/Bal
| |
| |
| |
|
| NB | 2 |
Lenoir, Deommodore 21/5
| 35 |
McCloud, Nick SF24
| |
| |
| |
|
| Special Teams |
| PT | 40 |
O'DONNELL, PAT SF24
| |
| |
| |
| |
|
| PK | 4 |
Moody, Jake 23/3
| |
| |
| |
| |
|
| LS | 46 |
PEPPER, TAYBOR SF20
| |
| |
| |
| |
|
| H | 40 |
O'DONNELL, PAT SF24
| |
| |
| |
| |
|
| KO | 4 |
Moody, Jake 23/3
| |
| |
| |
| |
|
| PR | 19 |
Cowing, Jacob 24/4
| 14 |
Pearsall, Ricky 24/1
| |
| |
| |
|
| KR | 1 |
Samuel Sr., Deebo 19/2
| 32 |
Taylor Jr., Patrick U/GB
| 31 |
Guerendo, Isaac 24/4
| |
| |
|
| Practice Squad |
| PS | 83 |
Owens, Terique WR
| 38 |
Mahoney, Jaylen PK
| 67 |
Alarcon, Isaac OT*
| 84 |
Gage, Russell WR
| 10 |
Bell, Ronnie WR
|
| PS | 75 |
Silvera, Nesta Jade DT
| 59 |
Wooten, Chandler LB
| 81 |
TAYLOR, TRENT WR
| 66 |
Nugent, Drake OC
| 26 |
Lucas, Chase CB
|
| PS | 96 |
Garvin, Jonathan DE
| 4 |
Mordecai, Tanner QB
| 47 |
White, DaShaun LB
| |
| |
|
| Reserves |
| IR | 3 |
WISHNOWSKY, MITCH PT^
| 28 |
Luter Jr., Darrell CB^
| 36 |
Robinson, Curtis LB^
| 78 |
Bartch, Ben OG^
| 48 |
Bethune, Tatum LB^
|
| IR | 23 |
McCaffrey, Christian RB^
| 24 |
Mason, Jordan RB^
| 30 |
ODUM, GEORGE S^
| 11 |
Aiyuk, Brandon WR
| 25 |
Mitchell, Elijah RB
|
| IR | 98 |
HARGRAVE, JAVON DT
| 55 |
FELICIANO, JON OC
| 90 |
Givens, Kevin DT
| |
| |
|
| PS/IR | 89 |
Pline, Mason TE^
| |
| |
| |
| |
|
| PUP | 95 |
Jackson, Drake DE
| |
| |
| |
| |
|
| SUS | 59 |
CAMPBELL SR., DE'VONDRE LB
| |
| |
| |
| |
|