| Active Players |
| 16 | Allar, Drew | QB | 03/08/2004 | 22 | 6' 05" | 228 | Penn State | PIT | 26 03 076 | R |
| 74 | Anderson, Spencer | OG/C | 06/07/2000 | 26 | 6' 05" | 305 | Maryland | PIT | 23 07 251 | 4 |
| 95 | Benton, Keeanu | DE | 07/17/2001 | 25 | 6' 03" | 309 | Wisconsin | PIT | 23 02 049 | 4 |
| 17 | Bernard, Germie | WR | 12/02/2003 | 22 | 6' 01" | 206 | Alabama | PIT | 26 02 047 | R |
| 94 | Black, Yahya | DT | 04/21/2002 | 24 | 6' 06" | 330 | Iowa | PIT | 25 05 164 | 2 |
| 9 | Boswell, Chris | PK | 03/16/1991 | 35 | 6' 02" | 185 | Rice | HOU FA | 14 CFA | 12 |
| 3 | Brisker, Jaquan | S | 04/20/1999 | 27 | 6' 01" | 204 | Penn State | CHI | 22 02 048 | 5 |
| 44 | Bruener, Carson | ILB | 06/05/2001 | 25 | 6' 01" | 226 | Washington | PIT | 25 07 226 | 2 |
| 60 | Cook, Dylan | OT | 01/11/1998 | 28 | 6' 06" | 305 | Montana | TB FA | 22 CFA | 3 |
| 35 | Dean, Jamel | CB | 10/15/1996 | 30 | 6' 01" | 206 | Auburn | TB | 19 03 094 | 8 |
| 13 | Dowdle, Rico | RB | 06/14/1998 | 28 | 5' 11" | 218 | South Carolina | DAL FA | 20 CFA | 7 |
| 26 | Echols, Brandin | CB | 10/16/1997 | 29 | 5' 11" | 179 | Kentucky | NYJ | 21 06 200 | 6 |
| 23 | Everette, Daylen | CB | 05/06/2004 | 22 | 6' 01" | 196 | Georgia | PIT | 26 03 085 | R |
| 76 | Fautanu, Troy | OT | 10/11/2000 | 26 | 6' 04" | 317 | Washington | PIT | 24 01 016 | 3 |
| 54 | Frazier, Zach | C | 08/29/2001 | 25 | 6' 03" | 310 | West Virginia | PIT | 24 02 051 | 3 |
| 88 | Freiermuth, Pat | TE | 10/25/1998 | 28 | 6' 05" | 258 | Penn State | PIT | 21 02 055 | 6 |
| 29 | Heidenreich, Eli | RB | 07/28/2003 | 23 | 6' 00" | 198 | Navy | PIT | 26 07 230 | R |
| 51 | Herbig, Nick | OLB | 11/21/2001 | 24 | 6' 02" | 240 | Wisconsin | PIT | 23 04 132 | 4 |
| 97 | Heyward, Cameron | DT | 05/06/1989 | 37 | 6' 05" | 295 | Ohio State | PIT | 11 01 031 | 16 |
| 56 | Highsmith, Alex | OLB | 08/07/1997 | 29 | 6' 03" | 242 | Charlotte | PIT | 20 03 102 | 7 |
| 67 | Hoffman, Brock | OG/C | 07/02/1999 | 27 | 6' 04" | 318 | Virginia Tech | CLE FA | 22 CFA | 5 |
| 55 | Holcomb, Cole | ILB | 07/30/1996 | 30 | 6' 01" | 240 | North Carolina | WAS | 19 05 173 | 8 |
| 21 | Homer, Travis | RB | 08/07/1998 | 28 | 5' 10" | 211 | Miami | SEA | 19 06 204 | 8 |
| 18 | Howard, Will | QB | 09/21/2001 | 25 | 6' 04" | 235 | Ohio State | PIT | 25 06 185 | 2 |
| 71 | Iheanachor, Max | OT | 10/19/2003 | 23 | 6' 05" | 321 | Arizona State | PIT | 26 01 021 | R |
| 92 | Jobity Jr., Kevin | DE | 10/06/2003 | 23 | 6' 04" | 300 | Syracuse | PIT FA | 26 CFA | R |
| 19 | Johnston, Cameron | PT | 02/24/1992 | 34 | 5' 11" | 194 | Ohio State | PHI FA | 17 CFA | 9 |
| 46 | Kuntz, Christian | LS | 04/13/1994 | 32 | 6' 01" | 228 | Duquesne | NE FA | 17 CFA | 6 |
| 62 | McCollum, Ryan | C | 03/04/1998 | 28 | 6' 05" | 300 | Texas A&M | HOU FA | 21 CFA | 4 |
| 66 | McCormick, Mason | OG | 05/25/2000 | 26 | 6' 04" | 315 | South Dakota State | PIT | 24 04 119 | 3 |
| 4 | Metcalf, DK | WR | 12/14/1997 | 28 | 6' 03" | 235 | Mississippi | SEA | 19 02 064 | 8 |
| 27 | Murphy-Bunting, Sean | CB | 06/19/1997 | 29 | 6' 00" | 195 | Central Michigan | TB | 19 02 039 | 7 |
| 31 | Nichols, Lew | RB | 08/16/2001 | 25 | 5' 10" | 220 | Central Michigan | GB | 23 07 235 | 1 |
| 37 | Nowakowski, Riley | FB | 06/30/2002 | 24 | 6' 02" | 250 | Indiana | PIT | 26 05 169 | R |
| 6 | Queen, Patrick | ILB | 08/13/1999 | 27 | 6' 00" | 230 | LSU | BAL | 20 01 028 | 7 |
| 5 | Ramsey, Jalen | S | 10/24/1994 | 32 | 6' 01" | 208 | Florida State | JAX | 16 01 005 | 11 |
| 8 | Rodgers, Aaron | QB | 12/02/1983 | 42 | 6' 02" | 223 | California | GB | 05 01 024 | 22 |
| 96 | Rubio, Gabriel | DT | 07/09/2003 | 23 | 6' 05" | 321 | Notre Dame | PIT | 26 06 210 | R |
| 2 | Rudolph, Mason | QB | 07/17/1995 | 31 | 6' 05" | 235 | Oklahoma State | PIT | 18 03 076 | 9 |
| 22 | Samuel Jr., Asante | CB | 10/03/1999 | 27 | 5' 10" | 180 | Florida State | LAC | 21 02 047 | 6 |
| 33 | Sawyer, Jack | OLB | 05/06/2002 | 24 | 6' 04" | 260 | Ohio State | PIT | 25 04 123 | 2 |
| 15 | Skowronek, Ben | WR | 06/27/1997 | 29 | 6' 03" | 224 | Notre Dame | LAR | 21 07 249 | 6 |
| 40 | Slaughter, Doneiko | CB | 11/08/2001 | 24 | 5' 11" | 190 | Arkansas | JAX FA | 25 CFA | 1 |
| 39 | Smith, Terell | CB | 07/27/1999 | 27 | 6' 00" | 207 | Minnesota | CHI | 23 05 165 | 4 |
| 28 | Spears-Jennings, Robert | S | 01/25/2004 | 22 | 6' 02" | 205 | Oklahoma | PIT | 26 07 224 | R |
| 85 | Tonyan, Robert | TE | 04/30/1994 | 32 | 6' 05" | 240 | Indiana State | DET FA | 17 CFA | 8 |
| 30 | Warren, Jaylen | RB | 11/01/1998 | 27 | 5' 08" | 215 | Oklahoma State | PIT FA | 22 CFA | 5 |
| 80 | Washington, Darnell | TE | 08/17/2001 | 25 | 6' 07" | 264 | Georgia | PIT | 23 03 093 | 4 |
| 90 | Watt, T.J. | OLB | 10/11/1994 | 32 | 6' 04" | 252 | Wisconsin | PIT | 17 01 030 | 10 |
| 10 | Wetjen, Kaden | WR | 03/09/2002 | 24 | 5' 09" | 196 | Iowa | PIT | 26 04 121 | R |
| 41 | Wilson, Payton | ILB | 04/21/2000 | 26 | 6' 04" | 242 | North Carolina State | PIT | 24 03 098 | 3 |
| Practice Squad |
| 65 | Baugh, Kyler | DT | 11/28/2001 | 24 | 6' 02" | 295 | Minnesota | NO FA | 24 CFA | 1 |
| 49 | Castro, Sebastian | S | 10/14/2000 | 26 | 5' 11" | 203 | Iowa | PIT FA | 25 CFA | 2 |
| 57 | Henry, KJ | OLB | 01/27/1999 | 27 | 6' 04" | 255 | Clemson | WAS | 23 05 137 | 3 |
| 82 | Hodgins, Isaiah | WR | 10/21/1998 | 28 | 6' 04" | 201 | Oregon State | BUF | 20 06 207 | 5 |
| 89 | Johnson, Brandon | WR | 07/26/1998 | 28 | 6' 02" | 208 | Central Florida | DEN FA | 22 CFA | 3 |
| 79 | Jones, Steven | OG/OT | 09/06/2000 | 26 | 6' 05" | 342 | Oregon | JAX FA | 24 CFA | 1 |
| 48 | Mapu, Marte | ILB | 11/08/1999 | 26 | 6' 02" | 230 | Sacramento State | NE | 23 03 076 | 4 |
| 81 | McRee, Lake | TE | 09/04/2002 | 24 | 6' 04" | 250 | USC | PIT FA | 26 CFA | R |
| 72 | Nester, Doug | OG/OT | 08/07/2000 | 26 | 6' 07" | 306 | West Virginia | MIN FA | 24 CFA | 1 |
| 93 | Otomewo, Esezi | DT | 12/30/1998 | 27 | 6' 05" | 282 | Minnesota | MIN | 22 05 165 | 4 |
| 50 | Roberts, Elandon | ILB | 04/22/1994 | 32 | 5' 11" | 238 | Houston | NE | 16 06 214 | 11 |
| 38 | Rush, Darius | CB | 02/22/2000 | 26 | 6' 02" | 198 | South Carolina | IND | 23 05 138 | 2 |
| 87 | Smith, Brandon | WR | 12/10/1998 | 27 | 6' 02" | 215 | Iowa | DAL FA | 21 CFA | 1 |
| 83 | Valdes-Scantling, Marquez | WR | 10/10/1994 | 32 | 6' 04" | 206 | South Florida | GB | 18 05 174 | 9 |
| 42 | Vigers, Car'lin | CB | 08/16/2000 | 26 | 6' 02" | 200 | Louisiana-Monroe | WAS FA | 25 CFA | 1 |
| 45 | Welschof, Julius | OLB | 03/12/1997 | 29 | 6' 07" | 265 | Charlotte | PIT FA | 24 CFA | 1 |
| 53 | Windmon, Jacoby | ILB | 08/20/2001 | 25 | 6' 01" | 250 | Michigan State | PIT FA | 24 CFA | 2 |
| Reserves |
| 85 | Burgess, Cole | WR | 02/11/2000 | 26 | 6' 00" | 200 | SUNY Cortland | CIN FA | 24 CFA | 1 |
| 68 | Driscoll, Jack | OT | 04/01/1997 | 29 | 6' 05" | 312 | Auburn | PHI | 20 04 145 | 6 |
| 73 | Dunker, Gennings | OG | 05/08/2003 | 23 | 6' 05" | 319 | Iowa | PIT | 26 03 096 | R |
| 25 | Elliott, DeShon | S | 04/21/1997 | 29 | 6' 01" | 210 | Texas | BAL | 18 06 190 | 9 |
| 31 | Hardy, Daequan | CB | 06/13/2001 | 25 | 5' 09" | 178 | Penn State | BUF | 24 06 219 | 1 |
| 99 | Harmon, Derrick | DE | 08/03/2003 | 23 | 6' 04" | 313 | Oregon | PIT | 25 01 022 | 2 |
| 84 | Hurleman, Max | WR | 10/19/2001 | 25 | 5' 11" | 200 | Notre Dame | PIT FA | 25 CFA | 1 |
| 69 | Joseph-Day, Sebastian | DE | 03/21/1995 | 31 | 6' 03" | 310 | Rutgers | LAR | 18 06 195 | 8 |
| 34 | Kent, Donte | CB | 09/28/2001 | 25 | 5' 10" | 189 | Central Michigan | PIT | 25 07 229 | 2 |
| 91 | Lee, Logan | DT | 06/12/2000 | 26 | 6' 05" | 291 | Iowa | PIT | 24 06 178 | 3 |
| 11 | Pittman Jr., Michael | WR | 10/05/1997 | 29 | 6' 04" | 223 | USC | IND | 20 02 034 | 7 |
| 14 | Wilson, Roman | WR | 06/19/2001 | 25 | 5' 11" | 192 | Michigan | PIT | 24 03 084 | 3 |